How do you calculate serial dilutions?
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You multiply the original concentration by the dilution factors for each dilution.
A serial dilution is any dilution in which the concentration decreases by the same factor in each successive step.
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In serial dilutions, you multiply the dilution factors for each step.
The dilution factor or the dilution is the initial volume divided by the final volume.
For example, if you add a 1 mL sample to 9 mL of diluent to get 10 mL of solution,
What is the dilution factor if you add 0.2 mL of a stock solution to 3.8 mL of diluent?
If you did the above dilution four times, what would be the final dilution factor?
Remember that serial dilutions are always made by taking a set quantity of the initial dilution and adding it successively to tubes with the same volume. So you multiply each successive dilution by the dilution factor.
You would transfer 0.2 mL from Tube 1 to 3.8 mL of diluent in Tube 2 and mix. Then transfer 0.2 mL from Tube 2 to 3.8 mL of diluent in Tube 3 and mix. Repeat the process until you have four tubes.
The dilution factor after four dilutions is
If the concentration of the original stock solution was 100 µg/µL, the concentration in Tube 4 would be
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100 µg/µL ×
Hope this helps.
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You might have a serial number or a redemption code depending on your product and where you purchased it.
Redemption Code (use this to obtain the serial number)
Find your redemption code on an insert card inside the box. The redemption code is a 24-digit alphanumeric code. For detailed instructions, see Redemption code help.
Find your serial number on the disc sleeve or on the product box. The serial number is a 24-digit numeric code.
Note: For Elements products, the serial number is on the Windows DVD sleeve.
The prepaid card contains a code you use to obtain your serial number and download your product. You'll find this code beneath the scratch-off foil on the back of the card.
For detailed instructions, see Redemption code help.
You can find your serial number on the Adobe Licensing Website.